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Anti-Green Fluorescent Protein Antibody说明书

更新时间:2023-10-24      点击次数:91

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产品信息

产品描述
Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions
来源
Chicken Polyclonal
亚型
IgY
应用
WB: 1:5000-1:10000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000
ELISA, Flow, ICC, IHC, WB
免疫原
Recombinant GFP expressed in Escherichia coli
缓冲液
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.
形式
Mixture of IgY fraction and affinity-purified antibodies
纯化方式
Chickens were immunized with recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.


货号品名规格品牌
78DA1179A-100uLAnti-Green Fluorescent ProteinAntibody100uLBIOHUB





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